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KMID : 0364819870250040304
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Korean Journal of Microbiology
1987 Volume.25 No. 4 p.304 ~ p.308
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¹H-NMR Spectroscopic Evidence on the Glycosidic Linkages of the Transglycosylated Products of Low-Molecular-Weight 1,4-β-D-Glucan Glucanohydrolase from Trichoderma koningli
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Maeng, Pil-Jae/맹필재
Kang, Sa-Ouk/Jeong, Choon-Soo/Hong, Soon-Woo/Hah, Yung-Chil/Rhee, Young-Ha/Kim, Jae-Heon/강사욱/정춘수/홍순우/하영철/이영하/김재현
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Abstract
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1
The mode of traaaglycosylatioa reaction observed during the action of low-molecularweight 1,4-j3-D-gluan glapaohydrolase (EC 3.2.1.4) purified from Tiiehodernw koningil ATCC 26113 was Investigated using 1H.NMR spectroscopy. The H-1 proton resonances were analysed. After reaction of the enzyme with cellotriose, the reaction products were separated by high performance liquid chromatography. H-1 resonaaces of the products were consisted with those of celloblose, cellotriase and cel otetraose, respectively. Therefore it was proved that all the reaction products formed by the action of the enzyme on Including trsntglycosytatiou products, possess only P-1,4-gbeasidic linkage(s)
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